FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8823996
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG11900.016.2313678
CGCGTAT7350.014.5522841
GGCGCGC9750.014.51281768
GTACGGC8300.013.29798717
GGCGGGC14200.013.11654613
CCCCGAT10150.012.9173771
CGGCAAT21950.012.7323121
CGCTATG18550.012.45811529
GTCGCTA19500.012.3820427
GTGCGAG15450.012.27858410
CGAACGT18150.011.97271727
ATCGGGC18950.011.8326546
TACCTAG18100.011.8165636
CCGACAT34800.011.699291
CGCGTTA18050.011.6628756
CGGGCGC16700.011.56606515
CGCGGGG6950.011.4182571
CGCGAAT16700.011.1566571
CGCAAAT18750.011.0409581
GGCGCAT18750.011.03733117
Produced by FastQC (version 0.11.2)