FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6881088
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA7150.011.58431956
CGCGTAT4904.6911737E-911.2665611
GTCCGCT5251.3782483E-810.5154561
CGGCAAT13950.010.3882541
GTCGAAT21750.010.1528541
GCCAGAT16900.010.0040731
CTCGGAT23600.09.7955821
CCGGCAT9900.09.7586631
GGGCGCT5059.7056E-79.56180711
GCGTGTA10200.09.4730252
TAACGCG8405.456968E-129.44892253
CGCGAAT8405.456968E-129.447481
CGCACAT6951.198714E-99.4327051
CGAGAAT34650.09.360351
CCTAGGC12200.09.329488
AATTGAG36750.09.2930895
CCGACAT16350.09.2854371
CGGAAAT29150.09.2325891
GCCCTAT8607.2759576E-129.22428510
GTCGATT18350.09.213561
Produced by FastQC (version 0.11.2)