FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14391597
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA35650.024.9704881
GTGTGCG31350.021.5643338
GTGCGAG34900.019.7661510
CGCGTAT21550.018.7329181
TGCGAGT38250.018.03499411
TGTGTGC39650.017.9201437
CTGACAT41450.017.5640541
CGGCAAT52750.017.268251
CGCTATG52300.016.61978329
GTCGCTA52400.016.39047627
AGGCGTT46950.016.24428254
GGCGGGC51050.016.215613
ACATCCG37250.016.12006655
CGTTCAG47500.016.05635857
CCGGCAT14950.015.9248291
TAGCCCG51000.015.82735834
TAGTCAT55650.015.8072346
GGCAATC57700.015.6051052
CGGGCGC54050.015.57082515
GTCCTAT41900.015.47964143
Produced by FastQC (version 0.11.2)