FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9462647
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167400.17690610248908153No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA123890.13092531085646542No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC117940.12463742967480451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT43900.026.4891511
GGCAGAC15250.026.4739441
GTGTGCG28850.024.1507998
GGCAATC49150.023.8652612
GCCAGTA13800.023.7545681
GCAATCG49150.023.7246273
GGCGGGC48750.023.56109613
TGTGTGC31800.022.1273257
GTGCGAG32350.021.96437610
CGGGCGC52050.021.93477415
CTGACAT33750.021.6752821
GGCGCAT53550.021.32035317
TAGCCCG52000.021.2291334
TGCGAGT33650.020.91081611
AGGCGTT52450.020.32962654
CATGCGT57300.020.22623821
ATGTGTG36550.019.8211566
CGTTCAG54000.019.68270757
GCAGACG20500.019.3532892
TCTAGCC57900.019.12526532
Produced by FastQC (version 0.11.2)