FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4101531
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA8600.023.2685471
GTGTGCG8200.019.7695458
GTGCGAG8550.018.15344610
CGCTATG11100.017.71230729
CTGACAT11600.016.0611081
TGCGAGT10150.015.29181811
TACGTGG10650.015.22997655
GTCGCTA12900.014.43867427
TGTGTGC11950.014.1429777
GCTACGT11950.013.86110353
CTACGTG12100.013.69010654
CCGGCAT4406.002665E-1113.3301781
ACATCCG10400.013.27326155
GCGATTT14150.013.1667031
TAGTCAT14900.012.7341076
GGCGCAG7050.012.72649153
CCGACAT21200.012.6941
GTCCTAT10350.012.66572543
CCGTTAA13300.012.44822920
TGAGGCG7500.012.42225650
Produced by FastQC (version 0.11.2)