FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48999860
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG98350.019.7096758
GTGCGAG114550.017.04271710
CGCTATG131250.016.39888429
GTCGCTA139450.015.4839827
CTTCCGA167600.015.1518231
TGCGAGT130550.014.9539911
GGCGGGC115450.014.93803713
CGCGTAT57400.014.7270341
CTGACAT158750.014.4533661
TGTGCGA137900.014.1319379
GACTGTC208750.013.9970644
GCCAGTA115400.013.7235981
CGGGCGC127850.013.59713315
CGGCAAT157800.013.3377911
TAGCCCG127150.013.32122734
TGTGTGC159100.013.072727
ATCGGGC152750.012.6920956
CCCTGTG159600.012.62860763
GGCGCAT139900.012.59855417
CCGACAT233850.012.5560571
Produced by FastQC (version 0.11.2)