FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24800268
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG38000.014.4320948
CGGCAAT71950.013.8592181
GGCGGGC50900.013.75605913
CGCGTAT23800.013.4827251
CGGGCGC52550.013.4554115
GTGCGAG42350.012.78680410
ATCGGGC62500.012.4192996
GGCAATC83000.011.88756752
CGCGGGG20650.011.8634211
GGCGCAT61950.011.80348717
TACGTGG56750.011.79711555
CTACGTG57050.011.795547554
TAGCCCG60050.011.71893334
GCGATTT83550.011.5633381
GCTACGT60500.011.23642353
GCAATCG82650.010.97775653
GCCAGTA53750.010.9771431
AATCGGG79700.010.9510765
TCGGGCG68550.010.9186287
TAGGGCG37100.010.8774669
Produced by FastQC (version 0.11.2)