FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4614367
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG3058.58563E-1015.8396141
GCCAGTA7850.014.0668451
CGCGTTA8050.013.71765656
GTGTGCG7050.013.6992578
CGCTATG11250.013.18419129
GTCGCTA11600.012.78625327
CCGGCAT4651.5279511E-1012.6157291
CGCGTAT4953.274181E-1112.5482661
GTGCGAG7950.012.14839810
CGAACGT8900.012.01446627
CGGCAAT13400.011.8459371
GCGAGAT7600.011.8052751
CCCCGAT5005.14774E-1011.7326281
GACATCG18550.011.7168123
GGCAATC13600.011.6690752
GGCGGGC8350.011.5664413
CGTACTG12300.011.49771522
CGCGCGT7250.011.42473565
TTAACGC9500.011.25980752
CCGACAT20200.011.10397151
Produced by FastQC (version 0.11.2)