FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20907297
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC241560.11553860836242963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA42700.022.6253071
CGGCAAT118150.022.0776271
GGCAATC126000.021.164492
GCAATCG126150.021.0843283
GGCGGGC130950.019.9921413
GTGTGCG73700.018.767228
AGGCGTT126700.018.76562554
GGCGCAT139350.018.76226617
TAGCCCG136850.018.55322534
CGGGCGC140800.018.54454815
CTGACAT78000.018.1365031
CATGCGT143000.018.11461321
TGACTTA135450.017.88089246
CGTTCAG135050.017.8357657
AGAGGCG139100.017.73693552
GTGCGAG79100.017.52962510
TCTAGCC149200.017.132632
ATCGGGC159650.017.1135276
TGTGTGC81700.017.0140657
GAGGCGT143400.016.74799553
Produced by FastQC (version 0.11.2)