FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8174700
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC129980.15900277686031292No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC99610.1218515664183395No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT96090.11754559800359647No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG87970.10761251177413239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT38450.037.5934643
CCTACTA38400.032.43097734
ACCTACT38000.032.31765433
TCTAGTT46000.031.866977
CTAATGC45450.031.49390821
ATGCTGG45550.030.59275224
ACACCTA40350.029.92250431
TCTAATG49400.029.46441720
TGGACAC43050.029.32487928
GACTATC40350.028.99289955
TAGTTGT50000.028.9727029
CACCTAC42700.028.92183932
TACTCTA51150.028.8664134
TAATGCT49900.028.82445722
TACCTCT50200.028.78856316
ATCTAAC43100.028.6576340
TGTTACC50200.028.5824413
GGACTGT37250.028.44309463
AATGCTG50650.028.12523823
ACTATCT45900.028.11159537
Produced by FastQC (version 0.11.2)