FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8403915
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGC11400.014.8280896
GGCGGGC8750.013.79698613
CGGCAAT18650.012.581581
CGGGCGC10000.012.41728615
TCGGGCG12350.012.00952157
GTCGAAT28250.011.9705111
TAGGGCG6109.094947E-1211.3090059
CGCGTAT5804.3655746E-1111.3039631
GCCGGAT12250.011.2675381
CGCGGGG7001.8189894E-1210.8450041
GCGGGCG10950.010.70998914
GGCGCAT12850.010.46852917
GCGGGGC3351.222743E-410.2984632
GGCAATC21400.09.834072
CGACAAT28800.09.4654161
GCGTGTA9900.09.41072952
AATCGGG18050.09.3651095
CGCGACT4855.9644535E-69.2492551
CCGGGAT15400.09.1868841
CCGACAT21450.09.1696481
Produced by FastQC (version 0.11.2)