FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5626200
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT28150.020.4703141
GGCAATC29450.019.6801932
GCAATCG29400.019.4786263
GGCGGGC28650.019.14224813
GTGTGCG17300.019.140168
GGCGCAT30800.017.80601717
GTGCGAG18450.017.76019110
CGGGCGC31100.017.63425615
AGGCGTT29550.017.40064654
GTCGCTA22400.017.09224927
TAGCCCG32200.017.03396434
CGCTATG22750.016.82929229
CATGCGT33800.016.53189521
GAGGCGT32400.016.50865753
TGCGAGT19850.016.50758411
TGTGTGC20900.016.5037217
CTGACAT22250.016.2834221
CGTTCAG31250.016.23319257
TGACTTA31950.015.87330146
AGAGGCG34150.015.86477652
Produced by FastQC (version 0.11.2)