FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11721157
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC193740.16529084970024718No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT147740.12604557724122284No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG128580.1096990681039423No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAG118910.10144902930657783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT74350.025.3831881
GCAATCG77700.024.8174133
GGCAATC78600.024.7970982
GGCGGGC85850.022.37857613
TATCTAG15500.020.919835
GGCGCAT91100.020.89961217
AGGCGTT86600.020.68167354
CGTTCAG88450.020.56166357
CGGGCGC92950.020.5578615
CTGACAT55100.020.4755691
TGACTTA85950.020.35184346
GTGTGCG56900.019.9434918
CATGCGT96000.019.9047221
TAGCCCG95600.019.41371534
TGTGTGC58550.019.3225527
AGAGGCG94800.019.25537752
CGCTATG61100.019.25016229
GAGGCGT93850.019.01004853
GGCAGAC12950.018.915961
GTTCAGT95550.018.78089558
Produced by FastQC (version 0.11.2)