FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12518232
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA781250.6240897276867852No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT525830.42005132993221406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGGC39800.021.31897213
CGGCAAT42650.021.1962761
CGGGCGC41700.020.18217515
GCAATCG47500.019.3188843
GGCGCAT43900.018.77792417
GGCAATC50300.018.4495282
TAGCCCG44000.018.11109234
CGCGTAT15700.017.3621981
GGCAGAC19400.016.8965931
GTGTGCG24350.016.8562978
GCGGGCG50550.016.64879814
AGGCGTT48550.016.49061254
CATGCGT50400.016.08242421
ATCGGGC57550.015.9448966
GTGCGAG26200.015.929358510
CGTTCAG50500.015.92237257
TCGGGCG57850.015.859617
CCTAGGC30950.015.379168
TGCGTCG55950.014.91900723
GAGGCGT53950.014.90321453
Produced by FastQC (version 0.11.2)