FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_84_old.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_84_old.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100935
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1730.1713974339921732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCAAC150.002349309469.027
GGCGATC150.002349309469.037
CCACGTC350.001341987939.4285743
TGGGAGA557.327648E-637.6363639
GGTTAAG450.004602940330.66666626
GCGAAAT605.387666E-428.751
CCGGCTT800.002881340221.56251
TCACATC950.007786314518.15789639
Produced by FastQC (version 0.11.2)