FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19307543
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT16750.016.2716121
CGGGCGC44100.015.9596515
GTGTGCG35000.015.5743388
CGGCAAT61300.015.4771331
GGCGGGC47900.014.9091213
TAGCCCG47300.013.93115534
ATCGGGC61550.013.1162096
GGCGCAT55000.013.04799117
GTGCGAG42600.012.95765510
TCGGGCG62000.012.8540417
GACTGTC69450.012.5680774
GGCGCGC29650.012.45013268
CCGGGGT14200.012.3908221
TGCGAGT46000.012.37491211
GGCCGAA55750.012.0672126
CATGCGT59300.012.04341621
GAGGCGT54900.012.00258553
GCAATCG73350.011.946873
CGCTATG49200.011.92064729
CGTTCAG54950.011.80334457
Produced by FastQC (version 0.11.2)