FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_83_old.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_83_old.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91867971
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT86650.017.3594631
GTGTGCG186750.015.8875238
CGGGCGC211500.014.66488915
GGCGGGC213200.014.49895113
CGGCAAT294850.014.1697221
TAGCCCG218250.013.75245734
CGCTATG244250.013.68691129
GTGCGAG219750.013.56440210
GCCAGTA233800.013.2658021
GACTGTC333100.012.8948034
CCGACAT306300.012.47991
GTCGCTA281750.012.08570727
TGCGAGT246050.012.05842611
GGCGCAT264200.011.90972917
GAGGCGT249900.011.90025653
ATCGGGC298150.011.7449656
CGTTCAG248900.011.74017257
TCGGGCG296000.011.5971097
ACCCGTA257150.011.30982432
AACGTCG305000.011.27758224
Produced by FastQC (version 0.11.2)