FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11566867
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT10800.016.930571
GTGTGCG20650.016.3730378
CGCTATG26200.015.27462429
CGCGGGG8000.014.2312631
GCCAGTA26650.013.7223391
CGGCAAT39350.013.6772671
CGGGCGC25650.013.5846915
TAGCCCG24900.013.16249234
GGCGGGC26600.012.84012613
GTGCGAG25000.012.83401110
CCGACAT35900.012.7813491
TAGGGCG20850.012.7410199
ACCTAGG23700.012.5191076
GTCGCTA34100.011.83720227
GGCCGAA32600.011.74689126
GACTGTC39500.011.7038584
TAAGCGG21800.011.55331120
TCGGGCG34500.011.500117
GGCGCAT30450.011.330517
GCAATCG43900.011.3166393
Produced by FastQC (version 0.11.2)