FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20344046
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT18100.019.6326391
GTGTGCG36200.016.392348
CGGCAAT59950.014.5021161
CGGGCGC44150.014.065783515
GGCGGGC45200.013.96784713
GTGCGAG44700.013.35227810
TAGCCCG45200.013.05192234
GCCAGTA46300.012.5183851
CCGACAT60650.012.2869181
TGCGAGT48600.012.280811
ATCGGGC60700.012.0495386
CGCTATG50750.011.82859629
GGCGCAT53600.011.77914517
TCGGGCG60550.011.5665277
CGCGGGG12600.011.2262131
ACCCGTA52100.011.12472832
TGTGCGA54900.011.0600519
CCGGCAT28550.010.9965211
GACTGTC71950.010.7408944
CCGGGAT38250.010.73335551
Produced by FastQC (version 0.11.2)