FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49395384
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG86450.017.4793998
CGCGTAT54100.016.962931
CGCTATG138700.015.42169729
GCCAGTA113350.014.7617111
GTGCGAG106600.014.46658210
TAGCCCG121400.013.98175134
CGGCAAT164500.013.7999181
GGCGGGC125300.013.79438313
GTCGCTA158150.013.45974627
ATCGGGC147550.013.2575156
CCGACAT216400.012.9772791
TGCGAGT121700.012.84172111
CGGGCGC136600.012.75474315
GGCGCAT142300.012.55932817
GACTGTC209500.012.416674
TCGGGCG156800.012.2553727
CCGGCAT62000.012.1305721
CGTTCAG133850.012.03684857
GCCAGCG127300.011.95158134
GTCCTAT125950.011.91534643
Produced by FastQC (version 0.11.2)