FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18098760
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG26100.014.9367738
GGCGGGC41200.014.90516613
CGGCAAT57300.014.7512191
CGCGTAT19100.013.9082921
CGGGCGC45800.013.63464815
GCCAGTA39400.013.3971251
TAGCCCG43250.013.32123234
GGCGCAT47400.013.10201817
CGCGAAT21450.013.0278921
GGCAATC67950.012.9470132
ATCGGGC52850.012.729456
GTGCGAG32600.012.27596510
TACGTGG46250.012.0095955
TCGGGCG55850.011.9221067
GCAATCG66250.011.8731993
GCTACGT48200.011.81002853
CTACGTG49550.011.69713954
GCGATTT66100.011.6913051
CGCTATG42900.011.66070329
GACTGTC68300.011.6178554
Produced by FastQC (version 0.11.2)