FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14530883
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG20850.015.88499458
CGCTATG31550.013.99663229
GTGCGAG25800.013.10455510
CGCGTAT12800.012.9374951
CCGACAT50150.012.5892271
GGCGGGC28050.012.29933313
CGGCAAT42550.012.2432371
GTCGCTA37500.011.59195427
TAGCCCG29050.011.40087134
GCCAGTA30950.011.1470071
ATCGGGC34100.011.0279676
CCGGCAT21450.010.6153791
CGGGCGC31850.010.507421515
TGCGAGT32300.010.36056911
TGTGCGA33000.010.3499249
CCGGAAC50900.010.3025491
TCGGGCG36200.010.2928847
GGCTGCC44300.010.27976343
GACTGTC49500.010.17585854
CCCTGTG42950.09.96027863
Produced by FastQC (version 0.11.2)