FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40074138
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC43600.013.29374315
CGCGTAT29100.012.8041081
CGGCAAT99750.012.4511111
CCGGCAT64750.011.4023021
GGCGGGC53000.011.32631513
GTGTGCG48400.011.26242358
TCGGGCG67250.010.7219597
TAGCCCG57450.010.38894334
CGCGCGT46300.010.35738765
ATCGGGC74300.010.2618526
CGCGGGG22900.010.0938731
CCGGGAT73950.010.0770651
CCGACAT104100.010.0086321
CGGGAAT82050.09.8811571
CGCTATG73700.09.73673329
CCGGAAC126950.09.7290141
CGGGCAT50200.09.2778761
GGCCGAA67400.09.16250826
GCGGGCG63650.09.16028414
TAGCGCG53650.09.13133862
Produced by FastQC (version 0.11.2)