FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11860758
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT9650.013.5854461
CGGGGAC8300.011.2228551
CGGCAAT30850.010.7357821
CCGGCAT18650.010.7291861
GGCGCGC12850.010.47073868
GTGTGCG16150.010.4674138
CGGGCAT16550.010.4229251
CGCGGGG7252.3646862E-119.9930711
CGCTATG21200.09.76403629
GCCAGTA27950.09.6278741
CGGGCGC15600.09.50994415
CGCGCGT14600.09.45198365
ATCGGGC22600.09.1592236
TAGCCCG16950.09.159183534
CCGGGAT23400.09.1409951
CCGACAT31500.08.9809221
TCGGGCG22300.08.6636117
GCCAGAT38450.08.6137551
GCGGAAT22850.08.6060971
AATCGGG31200.08.51435855
Produced by FastQC (version 0.11.2)