FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29066191
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA493520.16979176941347424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC34150.013.94187715
CGGCAAT72300.013.4087251
GTGTGCG37850.012.57856758
CGCGTAT22200.012.1216321
GGCGGGC41500.011.80470813
TAGCCCG40250.011.65703334
TCGGGCG48500.011.5947957
CGGGCAT38550.011.3657681
CCGGGGT19150.010.9895651
CGCGGGG17300.010.9682171
ATCGGGC54200.010.6301016
GCGGAAT51650.010.4869391
GCGGGCG47100.010.32819514
CCGGGAT54250.010.2387181
CGCGAAT27200.010.1470671
CCGACAT75950.09.7663011
GGCGCAT49800.09.69933717
CGGGAAT60800.09.5329021
CGCTATG52800.09.27833729
GCGATTT96900.09.2569731
Produced by FastQC (version 0.11.2)