FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77787175
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT54250.012.400871
CGGGCGC72800.011.94261915
CGGCAAT181000.011.1123851
GGCGGGC85150.010.21018213
CGGGCAT93450.09.4510041
TAGCCCG91950.09.41753134
CCGGGGT48000.09.4155861
CGGGAAT169250.09.3154711
GTGTGCG97450.09.240098
CGCGGGG39250.09.0534671
CCGGGAT144950.09.0444611
CCGACAT191900.09.04295351
CCGGCAT123350.09.0340131
TCGGGCG119850.08.9523987
ATCGGGC128550.08.8833016
CCGGAAC232850.08.7268271
GCGGGCG98350.08.66458414
GGAAAAT667200.08.3715931
CGCTATG132850.08.15429729
CCCGACT219100.08.140771
Produced by FastQC (version 0.11.2)