FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18178099
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA245270.1349260998083463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC23150.012.81647915
CGGCAAT49950.012.7086321
CGCTATG31200.012.38457529
CGCGTAT14050.012.0319561
ATCGGGC33800.011.840236
TAGCCCG26800.011.45702134
GTGTGCG25300.011.4545088
CGGGCAT26100.011.3677471
CCGACAT43900.011.316561
GGCGGGC26350.011.25992513
TCGGGCG32000.010.996847
CCGGGAT33600.010.88386251
CGGGAAT40500.010.4777151
CGCGGGG10900.010.1283791
ACCCGTA31550.09.84148932
GGCGCAT32950.09.63319617
GGCGCGC14400.09.58322268
CCGGCAT29850.09.3617531
GTCGCTA40800.09.30154227
GCGGGCG31900.09.19283514
Produced by FastQC (version 0.11.2)