FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19322849
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG33800.016.7397798
CGCGTAT19200.016.5311681
CGCTATG52900.015.19569429
CGGGCGC39750.014.66822915
CCGACAT64700.014.29051
GGCGGGC42250.013.79993113
GTCGCTA60750.013.62966727
TAGCCCG40600.013.51105134
GTGCGAG42800.013.30016610
CGGCAAT60500.013.1156371
AACGTCG65100.012.9839424
GGCTGCC63750.012.771667543
CCCTGTG63850.012.75163263
TAGGGCG31750.011.8440059
TCGGGCG54250.011.8286657
CGCGGGG13750.011.7926681
TGCGAGT48800.011.66490111
GGCGCAT50050.011.64968417
GGCCGAA56200.011.54095626
CCTAGGG35450.011.48387057
Produced by FastQC (version 0.11.2)