FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19991116
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT17550.016.5127491
CGGGCGC34050.016.21174815
TAGCCCG36400.014.88039334
GGCGGGC38650.014.28192113
CGGCAAT56800.013.8485331
CCGGGGT14200.013.6055761
GTGTGCG29500.012.6304868
TCGGGCG47600.012.3214067
ATCGGGC48750.012.2430866
GCGGGCG47450.011.56059914
GGCGCAT48250.011.51238617
CGCTATG44500.011.4740829
GGCCGAA49000.011.40621726
AATCGGG64800.011.0208415
GAGGCGT52150.010.98168253
CATGCGT53300.010.87482221
GTCCTAT43650.010.82810543
TAGGGCG29950.010.8279739
ACGGCAC53100.010.78521154
GCCAGCG44800.010.70416934
Produced by FastQC (version 0.11.2)