FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28278356
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC61350.015.40848815
GGCGGGC62950.015.40016713
TAGCCCG62400.014.98305334
CGGCAAT87200.014.63881
GTGTGCG47500.014.1631958
GCCAGTA67150.013.56371
CGCGTAT27700.013.3267491
TCGGGCG82950.012.6022037
ATCGGGC83500.012.5605786
GGCGCAT75150.012.48752217
CGCTATG73150.012.30956129
CGCGGGG22800.012.256611
TAGGGCG50150.011.90129
CCTAGGG52450.011.7083247
ACCTAGG51550.011.6450976
CCGACAT91550.011.6068021
TACGTGG67850.011.59308655
GCTACGT70600.011.48359953
GACTGTC97800.011.4647944
CATGCGT83800.011.4043521
Produced by FastQC (version 0.11.2)