FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26038344
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT73350.012.2760671
GGCGGGC42800.011.52681813
CGGGCGC42450.011.37841315
GTGTGCG31250.011.3712178
ATCGGGC49200.011.0091626
CGCGTAT27850.010.6534961
TCGGGCG51600.010.3633877
TAGCCCG45000.010.27324934
CCGGCAT35600.09.8848271
GGCGCAT52150.09.72523917
GCCAGTA55500.09.57296851
CGCGAAT34000.09.335291
CTACGTG53000.09.30841454
GCCGGAT47200.09.2828351
GCTACGT52600.09.18243553
CGCGCGT34950.09.180265
GGCGAAT65100.09.1681991
GTCGAAT97950.09.1577291
CGCTATG44150.09.142673529
CCGGAAC85100.09.0810781
Produced by FastQC (version 0.11.2)