FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37727930
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT33100.013.5497541
GGCGGGC56900.012.85398913
ATCGGGC68550.012.4814126
CGGGCGC59100.012.43423515
CGGCAAT104000.012.3402971
TCGGGCG71200.011.4838297
TAGCCCG64450.011.13410434
GTGTGCG43200.011.0208148
CCGGGGT55850.010.7483571
CGCGGGG37400.010.70045951
CCGACAT119450.010.4264651
GGCAATC127100.010.4232852
GCGAAAT153100.010.3431661
GCGGGCG71250.010.265285514
CGCTATG66400.010.235661529
GCGGAAT68900.010.2147321
GGCGCAT75700.010.11800817
AATCGGG98200.010.0127255
GTCGAAT149500.09.8768541
CCGGGAT75800.09.8310651
Produced by FastQC (version 0.11.2)