FastQCFastQC Report
Tue 31 Oct 2017
HWCGKBGX3_n01_ap_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGKBGX3_n01_ap_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66826778
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT57950.012.9783931
GTGTGCG80250.011.9513958
CGGCAAT182700.011.405551
GGCGGGC107650.011.08863913
CGGGCGC110100.010.87354315
ATCGGGC128950.010.7821036
TAGCCCG114400.010.4343434
CCGGGAT131400.010.3184631
TCGGGCG137350.010.0724357
CGCTATG122100.09.861135529
CCGGCAT95600.09.8519591
CGCGAAT85750.09.5754821
CCGACAT217000.09.38015651
CCGGGGT89550.09.3617821
GGCGCAT133500.09.35550517
CGCGGGG67150.09.350681
GCCGGAT128000.09.1370821
GCGATTT221250.09.0284481
GGCAATC232900.09.0064412
TACGTGG133350.08.97741655
Produced by FastQC (version 0.11.2)