FastQCFastQC Report
Wed 16 May 2018
HWCF7BGX5_n02_hmtest.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCF7BGX5_n02_hmtest.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410658196
Sequences flagged as poor quality0
Sequence length32
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACTCTGCGTTGATACCACTGCTTCCGC5487990.13363887664864724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGC2637850.017.9840226
GCTTCCG3118600.015.22104425
GTATCAA1659700.014.9844811
CCGCGGA388200.012.67804726
GGTATCA622550.012.2835491
GGCGTAC260900.011.1466881
CGTCGAA75800.010.61523722
TCAACGC2557050.010.2035143
GCGTACT518050.010.0370222
GTATTAG549500.010.0022721
ACGGTAG145150.09.8423919
TATCAAC2671700.09.7951251
GTATAGA547750.09.708781
ATCAACG2691150.09.6529682
TGCTTCC5174400.09.30240524
GAGTACG614100.09.2446091
CGCGGAC180200.09.23370625
TCCGCGG543350.09.2037225
GTATAAT941200.09.1244031
CAACGCA2848600.09.116864
Produced by FastQC (version 0.11.2)