FastQCFastQC Report
Wed 16 May 2018
HWCF7BGX5_n01_hmtest.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCF7BGX5_n01_hmtest.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410658196
Sequences flagged as poor quality0
Sequence length26
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTTT95589650.019.15353420
CTTTTTT108598500.019.06632620
GATTTTT48912500.018.99391619
GGATTTT27481450.018.9388318
GCTTTTT51311550.018.89743819
TATTTTT21007600.018.78521519
GGCTTTT27792700.018.78477118
TGTTTTT35596100.018.74704719
GTTTTTT166578700.018.74540920
TCTTTTT28004850.018.70177319
GGGATTT15090350.018.67474217
GTATTTT9728150.018.5562318
GGGCTTT15194150.018.48931317
TGGTTTT20617100.018.48821818
CGTTTTT18956800.018.4815119
AGTTTTT17954500.018.45842719
TGCTTTT10683300.018.45016718
GTGTTTT17036100.018.40989918
TGATTTT9951700.018.39351818
GCATTTT7648350.018.39351718
Produced by FastQC (version 0.11.2)