FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10681822
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG30000.039.4407211
GATCGGA35200.038.5447121
CACACGT31850.037.35959612
CACGTCT30950.036.99643314
AGCACAC33250.036.9386810
ACACGTC31200.036.84057213
ACGTCTG32200.036.11931215
CGTCTGA32750.035.5133916
AGAGCAC37050.035.27518
TCGGAAG40750.034.6135983
GAACTCC34650.033.2604221
ATCGGAA44600.031.9290372
CGGAAGA44750.031.6198884
TGAACTC38850.031.22846220
CCAGTCA33400.031.06219126
CAGTCAC31600.030.69472327
CTGAACT38950.030.51189219
CTCCAGT36250.030.05260524
AACTCCA45150.026.22475222
GAGCACA60050.022.3258259
Produced by FastQC (version 0.11.5)