FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH8782-i4h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH8782-i4h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6273879
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC908801.4485456286294334TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG117050.043.3822811
ACACGTC118350.042.9434313
CACACGT119150.042.5984512
CACGTCT119750.042.40447614
ACGTCTG120100.042.13103515
AGCACAC121100.042.0425610
GATCGGA119400.041.919321
CGTCTGA121850.041.67366816
ACTGACC120350.041.616232
GAGCACA122850.041.4802939
GTATGCC113450.041.4705545
TCCAGTC121700.041.4510325
GAACTCC122550.041.1848421
CCAGTCA122650.041.1306226
CAGTCAC123150.041.1101227
AGAGCAC124700.040.8832748
TCGGAAG125100.040.682883
CGTATGC115950.040.47970244
GTCTGAA125700.040.35032317
CTCGTAT110850.040.28908542
Produced by FastQC (version 0.11.5)