FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH8782-4h-Top2chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH8782-4h-Top2chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5630740
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC81330.1444392744115338TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG27050.035.1829915
CGTCTGA27050.035.09981516
GCACACG27100.034.5372311
GATCGGA27000.034.4246371
CACGTCT27900.033.707914
ACACGTC28000.033.5071613
CACACGT28200.033.26952412
GAACTCC29100.032.3980821
CCAGTCA30000.031.42529726
GAGCACA30050.031.2964679
TCCAGTC30450.031.18256225
CAGTCAC30200.030.91944127
AGCACAC30600.030.6604210
TCGGAAG30900.030.5833573
AGAGCAC31800.029.7864328
AGTCACC31350.029.5699228
GTCACCT31450.029.40435629
ACTCCAG31800.029.3642723
GTCTGAA32200.029.34888517
GTATGCC20900.027.77587545
Produced by FastQC (version 0.11.5)