FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH7797-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH7797-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9402587
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG10750.012.3525451
GCACACG12100.011.15633911
ACACGTC13300.010.82628713
CGTCTGA13850.010.55891816
ACGTCTG13550.09.63040415
CACACGT14150.09.53994812
CGATCTC12350.09.29102638
CACGATC14100.08.93595436
CACGTCT17150.08.6583614
CATCACG15950.08.32321433
GAGCACA19150.08.2240819
TCCAGTC16800.08.16953225
GATCTCG13800.08.15184739
AGCACAC17700.08.00798210
CAGTCAC17900.07.66773827
GTATGCC15900.07.641730345
CGTATGC17450.07.220839544
CCAGTCA18850.07.161744626
GAACTCC19300.07.111529421
AGAGCAC21650.06.96269468
Produced by FastQC (version 0.11.5)