FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH7797-input-resonic.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH7797-input-resonic.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6797083
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC8200.012.07329333
GCACACG7650.011.76404711
ACTTAGG7950.011.32095232
ACGTCTG9450.010.47537215
CACACGT9500.010.18349112
CGTCTGA9200.010.0263916
ACACGTC9000.09.99929213
CACGTCT9500.09.9465914
GAACTCC11400.08.48761521
AGCACAC11650.08.304255510
TTAGGCA12000.07.874674334
CAGTCAC11750.07.85125927
TAGGCAT12450.07.58993635
GATCGGA11900.07.37521741
TCCAGTC12300.07.317147325
GAGCACA13000.07.2688239
CCAGTCA13100.07.213866726
AGTCACT14800.06.68930628
GTCTGAA15200.06.66169317
CACTTAG14100.06.38313731
Produced by FastQC (version 0.11.5)