FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH7797-i3h-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH7797-i3h-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6152015
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC323330.5255676392206455TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC41100.041.11671429
TCACGCC42150.040.039430
GCACACG43150.039.7829511
AGTCACG43150.039.42405728
CACACGT44900.038.3820912
ACACGTC45300.037.94384813
ACGTCTG45200.037.92824615
GATCGGA44950.037.899711
CACGCCA45000.037.65358431
CACGTCT45650.037.65292714
AGCACAC46600.037.2238910
CGTCTGA45950.037.2112516
GTATGCC43400.036.395745
GAGCACA48150.036.072349
TATCTCG44700.036.03959739
CAGTCAC47400.035.84114527
CCAGTCA47900.035.6992926
TCCAGTC47950.035.61456325
GAACTCC49050.035.00105721
CGTATGC44650.034.9736344
Produced by FastQC (version 0.11.5)