FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH7797-i0h-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH7797-i0h-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5779364
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCC146850.2540937030441412TruSeq Adapter, Index 5 (98% over 51bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69430.120134326199215No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC62040.10734745207258099TruSeq Adapter, Index 5 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGCC60420.1045443754710726TruSeq Adapter, Index 5 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG77050.042.0782511
ACACGTC78400.041.41036613
CACACGT78400.041.38166812
GTCACAC78100.041.2838629
GATCGGA78650.041.0880931
ACGTCTG78900.041.0627515
CACGTCT79450.040.92008614
CGTCTGA80100.040.5318416
CACACAG80050.040.36251431
AGCACAC80900.040.0757610
GAGCACA81500.039.9463659
CAGTCAC81400.039.5269227
AGTCACA82100.039.32693528
AGAGCAC82800.039.210838
GAACTCC82250.039.14850221
CCAGTCA82700.038.7984326
TCCAGTC82850.038.7550125
CACAGTG83000.038.71040333
TCGGAAG85050.038.7032623
ACTCCAG83850.038.4014823
Produced by FastQC (version 0.11.5)