FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH7797-Myc-resonic.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH7797-Myc-resonic.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5647186
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC548710.9716520759188736TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG65200.042.37529811
ACACGTC66250.041.7376513
AGTCACG66050.041.59373528
CGATCAG66100.041.3913433
ACGTCTG66700.041.38860315
CACACGT66900.041.298512
GTCACGA66450.041.2417829
CACGTCT67650.040.74086814
GATCTCG63750.040.72784839
CGTCTGA67850.040.68709616
AGCACAC67900.040.62410
GATCGGA66900.040.5683251
CACGATC67750.040.48363531
GTATGCC67650.040.2448545
CAGTCAC68850.039.90184427
GAGCACA69600.039.6964049
TCCAGTC69200.039.63525
CGTATGC68950.039.61659644
AGAGCAC70750.039.1151058
TCGGAAG71100.038.956623
Produced by FastQC (version 0.11.5)