FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH7797-3h-Top2chip-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH7797-3h-Top2chip-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6119764
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC76590.12515188494196836TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG38400.038.1421811
ACGTCTG38750.037.27451315
GATCGGA38900.036.8509371
CACGTCT39950.036.4364614
ACACGTC40100.036.41237613
CACACGT40450.036.15292712
CGTCTGA40050.036.00843416
GAGCACA42100.034.8437469
AGCACAC42850.034.07608410
CCGATGT42300.033.88310633
GAACTCC42800.033.70113821
CAGTCAC42950.033.6322627
TCCAGTC43200.033.3858225
CACCGAT43900.032.80248331
AGAGCAC44700.032.7669758
CCAGTCA44100.032.60269526
AGTCACC44200.032.57957528
GTCACCG44100.032.55167429
CGATGTA44700.032.46524434
ACTCCAG44750.032.13202723
Produced by FastQC (version 0.11.5)