FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH7797-0h-Top2chip-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH7797-0h-Top2chip-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5992738
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG345120.575897027368792TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG47500.038.9816611
GATCGGA47550.038.4777871
ACGTCTG48250.038.42203515
CACGTCT48250.038.18857614
CACACGT49000.037.8798612
TCCAGTC49050.037.43124425
ACACGTC49600.037.4216313
CGTCTGA49850.037.143716
GAACTCC49750.037.08764321
AGCACAC50800.036.53796410
GAGCACA51350.036.0589839
CCAGTCA50800.036.05319626
CAGTCAC51250.035.69332527
CACAGTC51450.035.3370931
GTCACAG51850.035.19466829
AGAGCAC52950.035.1396378
CTCGTAT47800.034.97506344
TCGGAAG53300.034.8262063
TCGTATG48650.034.7799545
AGTCAAC51700.034.64192234
Produced by FastQC (version 0.11.5)