FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH7606-rep2-i3h-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH7606-rep2-i3h-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5178829
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG822721.5886216748998663TruSeq Adapter, Index 19 (97% over 40bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86300.16663998753386142No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53110.10255214064801137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG102100.042.5735911
GATCGGA101150.042.360451
ACACGTC103050.042.15846313
ACGTCTG102950.042.09014515
AGTCACG102800.042.04578428
CACGTCT103700.041.85081514
CACACGT103900.041.74860412
CGTCTGA104350.041.61168716
GATCTCG94900.041.5607141
CGATCTC94550.041.45319740
TCACGTG104150.041.4155730
GTCACGT104300.041.3763829
AGCACAC105300.041.343910
CACGTGA104150.041.32954831
GAGCACA105200.041.3190429
AGAGCAC105800.041.128448
CAGTCAC105700.040.99824527
TCGGAAG106300.040.873463
GTCTGAA107150.040.59239617
TCCAGTC106500.040.56234425
Produced by FastQC (version 0.11.5)