FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH7606-rep2-Top2chip-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH7606-rep2-Top2chip-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5845472
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG170480.29164454127913025TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG31300.037.52136611
CACACGT32000.036.34873612
ACGTCTG32450.035.42867715
ACACGTC33400.035.09457813
CACGTCT33000.034.9063814
GATCGGA32700.034.6192471
GAACTCC33300.034.59752721
AGCACAC34100.034.24249610
CGTCTGA33900.033.9796616
CAGTCAC34300.033.39004527
AGTCACA34400.033.2275728
GAGCACA35550.032.9091159
TCCAGTC35300.032.69939425
GTCACAT34900.032.6873429
TATCTCG28800.032.34206441
CCAGTCA36000.031.93884526
GTCTGAA36700.031.63755817
AGAGCAC37150.031.3709138
TCGGAAG37850.030.851233
ACTCCAG37900.030.57673323
Produced by FastQC (version 0.11.5)