FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH7606-rep1-i3h-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH7606-rep1-i3h-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5566279
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG455960.8191468663356617TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG58250.041.29316332
CGTCCGC58100.041.2828533
GCACACG59500.041.14158211
AGTCACG59850.040.2640828
CACGTCC59950.040.08468231
ACACGTC60550.039.9811713
GATCGGA61550.039.9275741
CACACGT61300.039.49200412
GTCCGCA60350.039.48167434
CCGCACA59850.039.39692336
AGCACAC62200.039.2833510
CGTCTGA61800.039.28170816
ACGTCTG62000.039.11870615
CACGTCT61950.039.0776414
GAGCACA62650.039.0370989
GTCACGT62200.038.70702429
TCACGTC62600.038.49564430
TCCGCAC62100.038.29557435
AGAGCAC64400.038.256158
CAGTCAC63350.038.14575627
Produced by FastQC (version 0.11.5)