FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH7606-rep1-Top2chip-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH7606-rep1-Top2chip-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6407164
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG441950.6897747583798386TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG52850.040.1433611
ACGTCTG53600.039.24616615
CACGTCT54250.038.90004714
ACACGTC54750.038.5858913
GATCGGA54250.038.5397381
GTCACAC54500.038.31447629
GAACTCC55200.038.0740821
CGTCTGA56150.037.58404516
CACACGT56750.037.30532512
TATCTCG54950.036.84953741
AGCACAC57600.036.83292810
GAGCACA57800.036.7444049
CAGTCAC57150.036.57695427
TCCAGTC57700.036.38229825
AGAGCAC58450.036.3742758
CACACTG57850.036.29051231
CCAGTCA58400.035.8311926
AGTCACA58650.035.68012628
TCGGAAG59850.035.6768383
ACTCCAG59300.035.3654723
Produced by FastQC (version 0.11.5)