Basic Statistics
Measure | Value |
---|---|
Filename | HWC7VAFXY_n01_AH5187-rep2-i3h-spike.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 5678605 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG | 109963 | 1.9364438977530571 | TruSeq Adapter, Index 16 (97% over 40bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCACACG | 13795 | 0.0 | 43.431316 | 11 |
GTCCCGA | 13080 | 0.0 | 43.276928 | 36 |
CGTCCCG | 13440 | 0.0 | 43.27278 | 35 |
GATCGGA | 14020 | 0.0 | 42.889687 | 1 |
ACCCGTC | 13675 | 0.0 | 42.81337 | 32 |
CCCGTCC | 13685 | 0.0 | 42.748444 | 33 |
TCACCCG | 13800 | 0.0 | 42.623478 | 30 |
ACACGTC | 14100 | 0.0 | 42.602417 | 13 |
CGTCTGA | 14060 | 0.0 | 42.5796 | 16 |
CACACGT | 14100 | 0.0 | 42.538593 | 12 |
CACGTCT | 14085 | 0.0 | 42.50403 | 14 |
ACGTCTG | 14060 | 0.0 | 42.499596 | 15 |
CCCGATC | 12220 | 0.0 | 42.4927 | 38 |
CACCCGT | 13900 | 0.0 | 42.268265 | 31 |
CCGTCCC | 13820 | 0.0 | 42.18173 | 34 |
AGCACAC | 14270 | 0.0 | 42.159065 | 10 |
GAGCACA | 14350 | 0.0 | 42.018105 | 9 |
GTCACCC | 14060 | 0.0 | 41.946934 | 29 |
TCCCGAT | 13120 | 0.0 | 41.909943 | 37 |
TCGGAAG | 14590 | 0.0 | 41.65295 | 3 |