FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH5187-rep2-i3h-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH5187-rep2-i3h-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5678605
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG1099631.9364438977530571TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG137950.043.43131611
GTCCCGA130800.043.27692836
CGTCCCG134400.043.2727835
GATCGGA140200.042.8896871
ACCCGTC136750.042.8133732
CCCGTCC136850.042.74844433
TCACCCG138000.042.62347830
ACACGTC141000.042.60241713
CGTCTGA140600.042.579616
CACACGT141000.042.53859312
CACGTCT140850.042.5040314
ACGTCTG140600.042.49959615
CCCGATC122200.042.492738
CACCCGT139000.042.26826531
CCGTCCC138200.042.1817334
AGCACAC142700.042.15906510
GAGCACA143500.042.0181059
GTCACCC140600.041.94693429
TCCCGAT131200.041.90994337
TCGGAAG145900.041.652953